Table II 
Known or predicted proteins encoded by S. aureus/S. epidermidis Twort–like phagesa

A5W protein name b	Length (amino acid residues)c	Homologue encoded by phaged: 	Degree of con–serva–tione (%)	Presence of homo–
logues in 
T, L, S, Ef	
Known or predicted function, or bases for name (references, comments)

Staph
1N	
Fi
200W	
P4W	
676Ż	
A3R	
MSA6	
G1	
ISP	
K	
Sb–1				
TreA 	99	+	+	+	+	+	+	150	130	+	177	99–100	–	terminal repeat-encoded protein (7,11)	
TreB 	61	+	+	+	+	+	+	231	131	+	+	96–100	–	terminal repeat-encoded protein (7,11)	
TreC 	95–96	+	+	+	+	+	+	156	132	+	178	35–100	–	terminal repeat-encoded protein (7,11)	
TreD 	95–108	+	+	+	+	+	+	158	133	117	179	68–100	–	terminal repeat-encoded protein (7,11)	
TreE 	97	+	+	+	+	+	+	154	134	+	180	97–100	–	terminal repeat-encoded protein (7,11)	
TreF 	85	+	+	+	+	+	+	175	135	+	181	99–100	–	terminal repeat-encoded protein (7,11)	
TreG 	79	+	+	+	+	+	+	183	136	+	182	99–100	–	terminal repeat-encoded protein (7,11)	
TreH 	115	+	+	+	+	+	+	125	137	118	183	100	–	terminal repeat-encoded protein (7,11)	
TreI 	112	+	+	+	+	+	+	128	138	+	–	100	T	terminal repeat-encoded protein (7,11)	
TreJ 	102	+	+	+	+	+	+	145	139	+	–	98–100	–	terminal repeat-encoded protein (7,11)	
TreK 	94–95	+	+	+	+	+	+	159	140	–	–	84–100	–	terminal repeat-encoded protein (7,11)	
TreL 	63	+	+	+	+	+	+	221	141	–	–	100	–	terminal repeat-encoded protein (7,11)	
    -	44	 -	-	-	-	+	+	312	142	–	–	98–100	–	terminal repeat-encoded protein (7,11)	
TreM	162	+	+	+	+	+	+	85	143	–	–	100	T, S	terminal repeat-encoded protein (7,11) homing endonuclease of HNH family (6)	
TreN 	52	+	+	+	+	+	+	+	144	–	–	98–100	T	terminal repeat-encoded protein (7,11)	
TreO 	43	+	+	+	+	–	+	297	145	–	–	98–100	–	terminal repeat-encoded protein (7,11)	
TreP 	107	+	+	+	+	+	+	135	146	–	–	94–100	–	terminal repeat-encoded protein (7,11)	
TreQ 	78	+	+	+	+	+	+	–	147	–	–	100	–	terminal repeat-encoded protein (7,11)	
TreR 	156	+	+	+	+	+	+	92	148	 +	–	100
	–	terminal repeat-encoded protein (7,11)	
TreS 	57	+	+	+	+	+	–	234	149	–	–	100	–	terminal repeat-encoded protein (7,11)	
+	56	+	+	+	+	+	–	247	+	+	–	100		? 	
TreT 	41–89	+	+	+	+	+	+	166	150	+	1	85–100	–	terminal repeat-encoded protein (7,11)	
TreU	59–73	+	+	+	+	+	+	226	151	+	2	28-100		terminal repeat-encoded protein (7,11)	
    +	26-33	+	+	+	+	+	+	450	+	+	+	97–100	–	?	
BofL 	78	+	+	+	+	+	+	184	152	 +	3	97–100	T	border ORF L protein, function unknown (7)	
  -	161	–	UboC	–	UboC	-	UboC	88	153	–	4	100	T	upstream of bof (11)	
-	135	 –	UboB	–	UboB	–	UboB	109	154	–	5	100	–	upstream of bof (11)	
       -	143	 – 	–	–	UboA	UboA	UboA	103	155	–	6	96–100	T	upstream of bof (11)	
gpORF005 	>56–57	 +	+	+	–	–	–	–	–	+	–				
gpORF006 	>84	 +	+	–	–	–	–	–	–	+	–				
MbpT 	63	+	+	–	+	+	+	224	156	+	7	98–100	–	putative membrane protein (7)	
MbpA 	161	+	+	+	+	+	+	87	157	1	8	99–100	–	membrane protein (11)	
gpORF008 	143	+	+	+	+	+	+	104	158	2	9	100	T		
gpORF009	180	 +	+	+	+	+	+	73	159	3	10	99–100	–		
gpORF010 	162	+	+	+	+	+	+	86	160	4	11	100	L, E		
gpORF011 	132	+	+	+	+	+	+	111	161	5	12	100	–		
gpORF012 	235	+	+	+	+	+	+	51	162	6	13	100	T, L, E	serine/threonine protein phosphatase I, by homology (2); phosphoesterase (7)   	
gpORF013 	184	+	+	+	+	+	+	70	163	7	14	100	–		
gpORF014 	105	+	+	+	+	+	+	138	164	+	15	100	T	virion component (1), putative tail protein (7)	
gpORF015 	182	+	+	+	+	+	+	71	165	8	16	98–100	T		
gpORF016 	72	+	+	+	+	+	+	201	166	+	17	99–100	–		
gpORF017 	64	+	+	+	+	+	+	218	167	+	18	98–100	–		
gpORF018	245	+	+	+	+	+	+	50	168	9	19	99–100	–		
gpORF019 	34	+	+	+	+	+	+	437	169	+	+	100	–		
gpORF020 	82	+	+	+	+	+	+	180	170	+	20	99–100	–		
gpORF021 	129	+	+	+	+	+	+	114	171	10	21	97–100	–		
gpORF022 	57	+	+	+	+	+	+	245	172	+	22	100	T		
gpORF023 	160	+	+	+	+	+	+	90	173	11	23	100	T		
gpORF024 	180	+	+	+	+	+	+	72	174	12	24	100	–		
gpORF025 	177	 +	+	+	+	+	+	77	175	13	25	100	–		
MbpP 	54	+	+	+	+	+	+	256	176	+	26	94–100	–	putative membrane protein (7)	
MbpR 	91–92	+	+	+	+	+	+	163	177	+	27	96–100	–	putative membrane protein (7)	
gpORF028 	281	+	+	+	+	+	+	38	178	14	28	99–100	–		
gpORF029	372	+	+	+	+	+	+	24	179	+/–	29	98–100	L	AAA family ATPase (2)	
gpORF030 	71–108	+	+	+	+	+	+	134	180	+	30	97–100	T		
gpORF031 	138–148	+	+	+	+	+	+	106	181	16	31	99–100	T		
HmzG 	100	+	+	+	+	+	+	149	182	17	32	100	–	homolog of MazG (7) 	
gpORF034 	62	+	+	+	+	+	+	228	183	+	33	98–100	T		
gpORF035 	53	+	+	+	+	+	+	259	184	+	34	100	T		
gpORF036	682	+	+	+	+	+	+	7	185	18	35	76–100	L, E		
gpORF038 	87	+	+	+	+	+	+	172	186	+	36	98–100	T	virion component (1,4)	
gpORF039 	57	 + 	+	+	+	+	+	+	+	+	37	98–100	T		
MbpB 	192	+	+	+	+	+	+	68	187	19	38	99–100	T	membrane protein (11)  	
gpORF041 	208	+	+	+	+	+	+	61	188	20	39	100	T		
Lig  	298	 +	+	+	+	+	+	32	189	21	40	98–100	L	DNA or RNA ligase 	
MbpU 	74	+	+	+	+	+	+	+	+	+	41	100	T	putative membrane protein (7) 	
Phr 	246	+	+	+	+	+	+	49	190	22	42	100	T	PhoH–related protein (2)  	
gpORF044 	204	+	+	+	+	+	+	63	191	23	43	100	T	virion component (1)	
Rbn 	141	+	+	+	+	+	+	96	192	24	44	100	T	ribonuclease (2)   	
gpORF046 	63	+	+	+	+	+	+	222	193	+	45	100	T		
gpORF047 	213	+	+	+	+	+	+	57	194	25	46	100	T		
gpORF048 	76	+	+	+	+	+	+	187	195	+	47	99–100	T, L, E		
gpORF049 	75	+	+	+	+	+	+	190	196	+	48	100	T		
Tgl 	210–230	+	+	+	+	+	+	54	197	26	49	100	T	putative transglycosylase (7)  	
gpORF051 	211	+	+	+	+	+	+	58	198	27	50	100	T		
MbpS 	263	+	+	+	+	+	+	44	199	28	51	100	–	putative membrane protein (7)	
MbpV 	102	+	+	+	+	+	+	146	200	29	52	100	–	putative membrane protein (7)	
LysKn/
LysKc (is)	495	 +(is)	+(is)	+(is)	+(is)	+(is)	+(is)	60/42 (is)	201/
203 (is)	30/32 (is)	53/55 (is)	99–100 	T	Endolysin, N–acetyl–muramoyl–L–alanine–amidase (2,10)   	
KsaI 	166	+	+	+	+	+	+	84	202	31	54	100	T, S	intron–encoded HNH endonuclease I–KsaI (2)	
HolA 	167	+	+	+	+	+	+	83	204	33	56	100	T	holin (2)   	
UphA 	61	+	+	+	+	+	+	233	205	+	57	100	T	upstream of hol	
Iro	72	 +	+	+	+	+	+	200	206	+	58	97–100	T, L	intergenic region ORF	
DmcB 	69	+	+	+	+	+	+	207	207	+	59	100	T	virion component (1), downstream of mbpC   	
DmcA 	110	+	+	+	+	+	+	209	208	+	60	99–100	T, L	downstream of mbpC  	
MbpC 	108	+ 	–	+	+	+	–	+	209	+	61	98–100	T, L	putative membrane protein (7)   	
MbpW 	88	+	+	+	+	+	+	169	210	+	62A	100	–	putative membrane protein (7)	
MbpD 	128	+	+	+	+	+	+	168	211	+	62	100	T, L	putative membrane protein (7)	
Dmd 	92	+	+	+	+	+	+	161	212	+	63	100	T, L	downstream of mbpD	
gpORF057 	136	+	+	+	+	+	+	133	213	34	64	100	T, L, E		
Ter 	605	 +	+	+	+	+	+ (is)	10	1	35	65	98-100	T, S, L, E	virion component, large terminase subunit (4)	
 –	323	 –	–	–	–	–	I-MsaI	–	–	–	–	–	T, E	intron–encoded protein  I-MsaI, function unknown (11)	
gpORF059 	273	+	+	+	+	+	+	40	2	36	66	98–100	T, L, E	virion component (4)	
gpORF59A	60	 +	+	+	+	+	+	235	3	+	67	100	–		
gpORF060 	159	+	+	+	+	+	+	91	4	37	68	100	T, L, E		
MbpF	397–403	 +	+	+	+	+	+	23	5	38	71	98–100	T	putative membrane protein (7); putative cell surface protein (2,6)	
MbpE 	116	+	+	+	+	+	+	120	6	39	73	99–100	T	membrane protein (11), see text	
gpORF063 	123	+	+	+	+	+	+	115	7	40	74	99–100	T, L, E		
Prt	563	+	+	+	+	+	+	14	8	41	75	99–100	T, L, S, E	virion component, portal protein (1,4)   	
Pro 	257	+	+	+	+	+	+	48	9	42	76	100	T, S, L, E	virion component, prohead protease (1)  	
gpORF066	316–318	 +	+	+	+	+	+	29	10	43	78	98–100	T, S, L		
Mcp 	463	+	+	+	+	+	+	16	11	44	79	100	T, S, L, E	virion component, major capsid protein (1,2,4) 	
gpORF068 	98	+	+	+	+	+	+	151	12	+	80	100	T, L, E		
gpORF069 	302	+	+	+	+	+	+	30	13	45	81	99–100	T, S, L, E	virion component, putative tail fiber protein (4)	
gpORF070 	158–292	+	+	+	+	+	+	34	14	46	82	99–100	T, S, L, E	virion component (1)	
gpORF071 	206	+	+	+	+	+	+	62	15	47	83	100	T, S, L, E		
gpORF072 	278	+	+	+	+	+	+	39	16	48	84	100	T, S, L, E	virion component, baseplate hub assembly protein (4)	
gpORF073 	71	+	+	+	+	+	+	202	17	+	85	99–100	T, L, E		
Tsp 	587	+	+	+	+	+	+	11	18	49	86	99–100	T, S, L, E	virion component, tail sheath protein (1,2,4)	
TmpA 	142	+	+(is)	+(is)	+(is)	+(is)	+	105	19	50	87	100	T, S, L, E	virion component, tail tube protein (1, 2, 4);  tail morphogenetic protein (7) 	
 –	467	 –	I-812I	I-812I	I-812I	I-812I	–	–	–	–	–	99–100	T	homologue of putative intron-encoded nuclease of phage 812 host range mutant (11,13)	
gpORF076	46	+	+	+	+	+	+	293	20	+	88	70–100	T		
gpORF077 	152	+	+	+	+	+	+	93	21	51	89	99–100	T		
MbpX 	64	+	+	+	+	+	+	215	22	+	89A	100	–	putative membrane protein (7)	
gpORF078 	103	+	+	+	+	+	+	141	23	52	90	100	T	virion component (1)	
gpORF079 	152	+	+	+	+	+	+	95	24	53	91	100	T, L, S, E		
TmpB 	178	+	+	+	+	+	+	74	25	54	92(f)	99–100	T, L, S, E	tail morphogenetic protein	
TmpC	1351–1352	 +	+	+	+	+	+	1	26	55	94	99–100	T, L, S, E	virion component (1,4); tape–measure protein (6); tail morphogenetic protein (7, 11) 	
TmpD	808	 +	+	+	+	+	+	5	27	56	96	99–100	T, L, S, E	tail morphogenetic protein (7)	
TmpE 	295	+	+	+	+	+	+	33	28	57	97	99–100	T, E	virion component, putative peptidoglycan hydrolase (4, 11), tail morphogenetic protein (7) 	
gpORF085 	848	+	+	+	+	+	+	4	29	58	98	99–100	T, L, S, E	glycerophosphoryl diester phosphodiesterase, by homology (2,4,6)	
gpORF086 	263	+	+	+	+	+	+	43	30	59	99	100	T, L, S, E	virion component (1,4)	
gpORF087 	174	+	+	+	+	+	+	78	31	60	100	99–100	T, L, E	virion component (4)	
BmpA 	234	+	+	+	+	+	+	52	32	61	101	100	T, L, S, E	virion component, putative baseplate wedge sububit, by homology (4); baseplate morphogenetic protein (7)	
BmpB 	348	+	+	+	+	+	+	27	33	62	102	100	T, L, S, E	virion component (1,4); baseplate morphogenesis protein, by homology (7)	
TmpF 	1019	+	+	+	+	+	+	3	34	63	103	99–100	T, L, S, E	tail morphogenetic protein F, by homology (7) 	
BmpC 	173	+	+	+	+	+	+	79	35	64	104	100	T, L, S, E	virion component (1,4), baseplate morphogenetic protein C, by homology (7)	
TmpG	1152	+	+	+	+	+	+	2	36	65	105	99–100	T, L, S, E	virion component (1,4); tail morphogenetic protein, by homology (7, 11)	
gpORF093 	52	+	+	+	+	+	+	262	37	+	106	100	T, L, S, E		
gpORF094 	640	+	+	+	+	+	+	8	38	66	107	99–100	T, L	virion component (1,4)	
gpORF095 	124	+	+	+	+	+	+	117	39	67	108	100	T	virion component (1)	
gpORF096 	458	+	+	+	+	+	+	17	40	68	109	99-100	T, L	virion component (1,4)	
DhlA 	582	+	+	+	+	+	+	12	41	69	110	99–100	T, L, S, E	DNA helicase A, by homology (2, 7)	
gpORF098 	537	+	+	+	+	+	+	13	42	70	111	100	T, L, E	Rep protein, by homology (2, 7) 	
DhlB 	480	+	+	+	+	+	+	15	43	71	112	99–100	T, L, S, E	putative DNA helicase B, by homology (2, 7)	
RncA 	345	+	+	+	+	+	+	28	44	72	113	100	T, L, S, E	recombination nuclease A; similar to putative exonuclease SbcD (2, 7) 	
gpORF102 	125	+	+	+	+	+	+	110	45	73	114	100	T	 	
RncB 	639	+	+	+	+	+	+	9	46	74	115	100	T, L, S, E	recombination nuclease B; putative exonuclease, by homology (2, 7) 	
Asf 	198	+	+	+	+	+	+	67	47	75	116	100	T, L, E	anti–sigma factor (8) 	
Pri	355	 +	+	+	+	+	+	26	48	76	+(fN)	98–100	T, L, S, E	DNA primase, by homology (2)   	
gpORF106 	112	+	+	+	+	+	+	127	49	+	119	100	–		
gpORF107 	150	+	+	+	+	+	+	98	50	77	120	98–100	T, L, E		
gpORF108 	202	+	+	+	+	+	+	64	51	78	121	99-100	T, L, S, E	homolog of T5 phage D14 protein (2), resolvase, by homology (4,7)	
NrdI 	143	+	+	+	+	+	+	102	52	79	122	100	T, L	ribonucleotide reductase stimulatory protein, NrdI, by homology (2,6) 	
NrdE	704	 +	+	+	+	+	+	6	53	80	123	99-100	T, L, S, E	ribonucleotide reductase large (alpha) subunit, by homology (2,6)	
NrdF	349	+	+	+	+	+	+	25	54	81	124	100	T, L, S, E	ribonucleotide reductase minor subunit, by homology (2,6)	
gpORF112 	109	+	+	+	+	+	+	130	55	82	125	98-100	–		
Tio 	106	+	+	+	+	+	+	129	56	83	126	99-100	T, L	thioredoxin like protein, by homology (2) 	
gpORF114 	198	+	+	+	+	+	+	66	+	84	127	100	T, L, E		
Trf 	101	+	+	+	+	+	+	147	57	85	128	100	T, L, S, E	transcription factor (7,11)  	
PolA (2is) 	1008-1072	+
(2is)	+
(2is) 	+
(2is)	+
(2is)	+
(2is) 	+
(2is) )	35/19/37 (2is) 	59/61/63 (2is) 	86/88/90 (2is) 	129/
129b/129c
(2is) 	99–100	T, L, S, E	DNA–directed DNA  polymerase A; related to bacterial PolA polymerases (2,6,7)   	
I–KsaII 	170	+	+	+	+	+	+	81	60	87	130	100	–	Intron–encoded protein I–KsaII (2), putative endonuclease (4)	
I–KsaIII	269	 +	+	+	+	+	+	41	62	89	132	99–100	T, S	intron–encoded protein I–KsaIII, putative HNH endonuclease (2)	
gpORF121 	80	+	+	+	+	+	+	181	64	+	135	99–100	–		
gpORF122 	160	+	+	+	+	+	+	89	65	91	136	100	T, L, E		
gpORF123 	296–423	+	+	+	+	+	+	20	66	92	137	100	T, L, E		
Rec	418	 +	+	+	+	+	+ (is)	21	67	93	138	100	T, L, E	putative repair recombinase (2); homolog of bacterial RecA (6,7)   	
 –	215	 –	–	–	–	–	I-MsaII	–	–	–	–	–	–	putative intron-encoded endonuclease I-MsaII, by homology (11)	
gpORF125 	117	+	+	+	+	+	+	121	68	+	139	100	T, L, E		
Sig 	220	+	+	+	+	+	+	56	69	94	140	100	T, L, S, E	RNA polymerase sigma factor, by homology (2,6)	
gpORF127 	210	+	+	+	+	+	+	59	70	95	141	100	T, L, E	virion component (1,2,4), putative tail morphogenetic protein (3,11)	
TmpH 	73–170	+	+	+	+	+	+	80	71	96	142	32–99	T, E	virion component (1,2,4); tail morphogenetic protein H, by homology (7,11) (In MSA6 a deletion of 3' tmpH end and 5' tmpI end caused a formation of a short fusion product)	
TmpI 	75	+	+	+	+	+	–	189	72	+	143	100	T	virion component (1,2); tail morphogenetic protein I, by homology (7, 11) 	
gpORF130 	86	+	+	+	+	+	+	174	73	+	144	100	T		
gpORF131 	251	+	+	+	+	+	+	46	74	97	–	97–100	T		
	    >152	–	–	–	–	–	–	–	–	–	+				
	    >106	–	–	–	–	–	–	–	–	–	+				
gpORF132 	416	+	+	+	+	+	+	22	75	98	147	100	T, L, E	putative metallophosphoesterase (6)	
MbpG 	122	+	+	+	+	+	+	118	76	99	148	100	T, L	putative membrane protein (7)	
gpORF134 	103	+	+	+	+	+	+	143	77	100	149	100	–		
gpORF135 	178	+	+	+	+	+	+	75	78	+	150	100	T, L, E		
gpORF136 	255	+	+	+	+	+	+	45	79	101	151	100	T, L, E		
gpORF137 	148	+	+	+	+	+	+	99	80	102	152	100	T, L, E		
gpORF138 	287	+	+	+	+	+	+	36	81	+	153	100	T, L, E		
gpORF139	243	 +	+	+	+	+	+	47	82	103	154	100	T, L, S, E		
gpORF140 	152	+	+	+	+	+	+	94	83	104	155	99–100	T, L, E	virion component (1)	
gpORF141 	147	+	+	+	+	+	+	100	84	105	156	100	T, L, E		
gpORF142 	234	+	+	+	+	+	+	53	85	106	157	99–100	T		
MbpH 	132	+	+	+	+	+	+	108	86	107	158	100	T	putative membrane protein (7)	
PufA	20	+	+	+	+	+	+	+	87	+	+	100	–	peptide of unknown function (11)	
gpORF144 	80	+	+	+	+	+	+	182	88	+	+	100	–		
gpORF145 	54–156	+	+	+	+	+	+	252	89	108	+	100	–	see text	
gpORF146 	59	 +	+	+	+	+	+	+	+	–	–	98–100	–		
Sci (gpORF147) 	58	+	+	+	+	+	+	240	90	–	–	100	–	DNA sliding clump inhibitor, arrest of S. aureus  DNA synthesis (7,9)	
MbpJ	177	+	+	+	+	+	+	76	91	–	–	100	–	putative membrane protein (7)	
MbpY 	47–82	+	+	+	+	+	+	–	92	–	–	100	–	putative membrane protein (7)  	
gpORF149 	58	+	+	+	+	+	+	241	93	–	–	91–100	T		
gpORF150 	98	+	+	+	+	+	+	152	94	–	–	100	–		
MbpK 	63	+	+	+	+	+	+	219	95	–	–	98-100	–	putative membrane protein (7)	
gpORF152 	122	+	+	+	+	+	+	119	96	–	–	97–100	–		
gpORF153 	115	+	+	+	+	+	+	124	97	–	–	100	–		
MbpI 	92	+	+	+	+	+	+	162	98	–	–	100	–	putative membrane protein (7)	
gpORF155 	101	+	+	+	+	+	+	140	99	–	–	100	T		
gpORF156 	116	+	+	+	+	+	+	122	100	–	–	100	–		
gpORF157 	200-381	+	+	+	+	– 	+	65	101	–	–	49-100	–	see text 	
gpORF158 	59	+	+	+	+	–	+	237	102	–	–	98–100	–		
PufB	22	 + 	+	+	+	–	+	+	103	–	–	100	–	peptide of unknown function	
PufC	24	 +	+	+	+	–	+	+	104	–	–	100	–	peptide of unknown function	
MbpM 	133	+	+	+	+	–	+	107	105	–	–	100	–	putative membrane protein (7)	
gpORF160	86–97	 +	+	+	+	–	+	173	106	–	–	55–100	E		
MbpL 	95	+	+	+	+	–	+	157	107	–	–	100	–	putative membrane protein (7)	
gpORF162 	38	+	+	+	-	–	+	362	108	–	–	100	–		
gpORF163 	87	+	+	+	+	–	+	170	109	–	–	100	–		
MbpZ 	59	+	+	+	+	–	+	236	110	–	–	100	–	putative membrane protein (7)	
gpORF164 	87	+	+	+	+	–	+	171	111	–	–	100	–		
gpORF165 	105	+	+	+	+	–	+	137	112	–	–	100	–		
gpORF166 	226	+	+	+	+	–	+	55	113	–	–	100	L	see text	
MbpN 	52	 +	+	+	+	–	+	263	114	+	160A	100	–	putative membrane protein (7)	
gpORF168 	66	+	+	+	+	–	+	211	115	+	161	100	–		
MbpO 	96	+	+	+	+	–	+	155	116	+	162	100	–	putative membrane protein (7)	
gpORF170 	102	 +	+	+	+	–	+	144	117	109	163	100	–		
Rpp 	302	+	+	+	+	–	+	31	118	110	164	100	L	ribose–phosphate pyrophosphokinase, by homology (2, 7)  	
NadV 	381–489
	+	+	+	+	+	+	NadV	119	119	165	98–99	L	virion component (1); putative nicotinamide phosphoribosyl transferase NadV, see text (2,6,7)	
gpORF173 	81	+	+	+	+	+	+	178	120	+	+/–	86-100	–		
gpORF174 	130–131	+	+	+	+	+	+	113	121	112	166	90–100	–		
gpORF175 	73	+	+	+	+	+	+	194	122	–	167	100	–		
        –	83	–	–	–	–	–	–	–	–	+
OsfA	–			ORF one hundred seventy five exchanger A (7)	
        – 	98	–	–	–	–	–	–	–	–	+
OsfB	–			ORF one hundred seventy five exchanger B; homolog of TreA, TreC, TreD and TreE (7)	
gpORF176 	103	+	+	+	+	+	+	142	123	113	168	86–100	–		
gpORF177 	169	+	+	+	+	+	+	82	124	–	169	100	T		
        – 	99	–	–	–	–	–	–	–	–	114	–		SP10	(homolog of Bacillus phage SP10  ORF42 product) (11,12)	
gpORF178 	109	+	+	+	+	+	+	131	125	–	170	99–100	T		
        – 	75	–	–	–	–	–	–	–	–	+
Ose				ORF one hundred seventy eight exchanger (7)	
gpORF179 	64-69	+ 	+	+	+	+	+	-	126	–	171	86-100	–		
gpORF180 	104	+	+	+	+	+	+	139	127	–	172	100	–		
        –	117	–	–	–	–	–	–	–	–	115	–				
        –	128	–	–	–	–	–	–	–	–	116	–				
gpORF181	62	+	+	+	+	+	+	225	128	–	173	100	S		
       BofR	33	 +	+	+	+	+	+	445	129	–	+	100	–	Border ORF R protein,  function unknown (7, 11)	

aThe function assignment of certain proteins is based on the following literature sources: 1, Eyer et al. (2007); 2, O’Flaherty et al. (2004); 3, Kilcher et al. (2010); 4, Vandersteegen et al. (2011); 5, Paul et al. (2011b); 6, Kwan et al. (2005); 7, Ulatowska et al. (in preparation); 8, Debhi et al. (2009); 9, Belley et al. (2006); 10, O’Flaherty et al. (2005a); 11, this work; 12, Yee et al. (2011); 13, Kasparek et al., (2007). Light-grey shaded rows indicate protein products of genes that are split in certain phages. Genes that are split by introns are indicated with (is). Proteins of some phages which are shorter homologues of corresponding proteins of other phages and are apparent products of split genes are listed below their longer counterparts and are indicated with >, prior to the number of their amino acid residues.
bProtein names and ORF numbers are as in Ulatowska et al. (in preparation). Names of proteins that are conserved in all S. aureus/S. epidermidis Twort–like phages are in bold.
cRanges of protein lengths indicate the shortest and the longest version of each protein in S. aureus/S. epidermidis Twort–like phages.
d Homologues of A5W phage proteins that are designated by the names corresponding to those of A5W proteins, in the GenBank files of phages Staph1N, Fi200W, P4W, 676Ż, A3R, MSA6 (see Table 1) are designated with +. Proteins of phages G1, ISP, K and Sb–1 that are annotated in the GenBank files of genomic sequences of these phages are designated with the relevant ORF numbers or names. Homologues of A5W proteins of the latter phages that are not annotated in the relevant GenBank files are designated with +. Their identification was based on homology searches and on predictions of the MetaGeneAnnotator (Noguchi et al., 2006, 2008). Predicted products of genes that do not match with MetaGeneAnnotator predictions are indicated with a question mark. Proteins that are missing in proteomes of certain phages are designated by – . Dark-grey shaded fields indicate genome regions that are deleted in certain phages. For the purpose of comparisons, amino acid sequences of homologous proteins that differed from each other due to annotation errors or differences in the assignments of start codons in DNA, despite identical gene sequences, were unified according to the annotations in the GenBank file of phage A5W sequence. Products of genes which may possibly contain errors in their sequences deposited in GenBank, are indicated with +/–. ORFs that are annotated in the sequences of phages G1 and ISP deposited in GenBank, but do not match with predictions of MetaGeneAnnotator were not included in the Table.
e Degree of conservation indicates the range of identities, in percent, between the least and the most homologous orthologues, as calculated based on the comparison of all orthologues with the shortest of them.
f T, L, S and E correspond to Staphylococcus phage Twort, Listeria phage A511 or P100, Bacillus phage SPO1 and Enterococcus faecalis phage phiEF24C, respectively. Homologies are indicated according to Stewart et al. (2009a) and Ulatowska et al. (in preparation).