Malecka, Kamila and Grabowska, Iwona and Radecki, Jerzy and Stachyra, Anna and Góra-Sochacka, Anna and Sirko, Agnieszka and Radecka, Hanna (2013) Electrochemical Detection of Avian Influenza Virus Genotype Using Amino-ssDNA Probe Modified Gold Electrodes. Electroanalysis, 25 (8). pp. 1871-1878. ISSN 1040-0397
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Official URL: http://onlinelibrary.wiley.com/doi/10.1002/elan.20...
Abstract
A DNA biosensor for the detection of specific oligonucleotide sequences of Avian Influenza Virus type H5N1 has been proposed. The NH2-ssDNA probe was deposited onto a gold electrode surface to form an amide bond between the carboxyl group of thioacid and the amino group from ssDNA probe. The signals generated as a result of hybridization were registered in square wave voltammetry and electrochemical impedance spectroscopy in the presence of [Fe(CN)6]3�/4� as a redox marker. The genosensor is capable to determine 20-mer and 180-bp (PCR products) oligonucleotides complementary sequences with detection limit in the fM range. The genosensor displays good selectivity and sensitivity. The 20-mer as well as 180-bp oligonucleotides without a complementary sequence generate very low signal.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology |
| ID Code: | 589 |
| Deposited By: | dr Anna Gora-Sochacka |
| Deposited On: | 11 Apr 2014 12:20 |
| Last Modified: | 11 Apr 2014 12:20 |
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