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Proper functioning of the GINS complex is important for the fidelity of DNA replication in yeast

Grabowska, Ewa and Wronska, Urszula and Denkiewicz, Milena and Jaszczur, Małgorzata and Respondek, Aleksandra and Alabrudzinska, Malgorzata and Suski, Catherine and Makiela-Dzbenska, Karolina and Jonczyk, Piotr and Fijalkowska, Iwona J. (2014) Proper functioning of the GINS complex is important for the fidelity of DNA replication in yeast. Molecular Microbiology, 92 (4). pp. 659-680. ISSN 1365-2958

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Official URL: http://onlinelibrary.wiley.com/doi/10.1111/mmi.125...

Abstract

The role of replicative DNA polymerases in ensuring genome stability is intensively studied, but the role of other components of the replisome is still not fully understood. One of such component is the GINS complex (comprising the Psf1, Psf2, Psf3 and Sld5 subunits), which participates in both initiation and elongation of DNA replication. Until now, the understanding of the physiological role of GINS mostly originated from biochemical studies. In this article, we present genetic evidence for an essential role of GINS in the maintenance of replication fidelity in S. cerevisiae. In our studies we employed the psf1-1 allele (Takayama et al., 2003) and a novel psf1-100 allele isolated in our laboratory. Analysis of the levels and specificity of mutations in the psf1 strains indicates that the destabilization of the GINS complex or its impaired interaction with DNA polymerase epsilon increases the level of spontaneous mutagenesis and the participation of the error-prone DNA polymerase zeta. Additionally, a synergistic mutator effect was found for the defects in Psf1p and in the proofreading activity of Pol epsilon, suggesting that proper functioning of GINS is crucial for facilitating error-free processing of terminal mismatches created by Pol epsilon.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Laboratory of Mutagenesis and DNA Repair
ID Code:689
Deposited By: M.Sc. Ewa Grabowska
Deposited On:14 May 2014 09:54
Last Modified:08 Mar 2018 15:33

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