Kurzątkowska, Katarzyna and Mielecki, Marcin and Grzelak, Krystyna and Verwilst, Peter and Dehaen, Wim and Radecki, Jerzy and Radecka, Hanna (2014) Immobilization of His-tagged kinase JAK2 onto the surface of a plasmon resonance gold disc modified with different copper (II) complexes. Talanta, 130 . pp. 336-41. ISSN 1873-3573
|
PDF
544kB |
Official URL: http://www.sciencedirect.com/science/article/pii/S...
Abstract
New surface plasmon resonance (SPR) sensing platforms which consists of copper (II) complexes of a pentetic acid thiol ligand (DPTA-Cu(II)) and of a thiol derivative of dipyrromethene (DPM-Cu(II) created on the surface of gold SPR disc were applied to oriented immobilization of His-tagged Janus kinase 2 (GST-His6-JAK2). This method is based on the covalent bond formation between histidine from a His-tag chain of a protein and Cu(II) centres from the complexes. The kinetic and thermodynamic parameters of the oriented immobilization of GST-His6-JAK2 protein to DPTA-Cu(II) and DPM-Cu(II) complexes attached to the Au surface of a SPR disc were discussed.
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QC Physics Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Department of Protein Biosynthesis |
| ID Code: | 790 |
| Deposited By: | dr Marcin Łukasz Mielecki |
| Deposited On: | 07 Nov 2014 10:58 |
| Last Modified: | 07 Nov 2014 10:58 |
Repository Staff Only: item control page
