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Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization

Kozakova, Hana and Schwarzer, Martin and Tuckova, Ludmila and Srutkova, Dagmar and Czarnowska, Elzbieta and Rosiak, Ilona and Hudcovic, Tomas and Schabussova, Irma and Hermanova, Petra and Zakostelska, Zuzana and Aleksandrzak-Piekarczyk, Tamara and Koryszewska-Bagińska, Anna and Tlaskalova-Hogenova, Helena and Cukrowska, Bozena (2016) Colonization of germ-free mice with a mixture of three lactobacillus strains enhances the integrity of gut mucosa and ameliorates allergic sensitization. Cellular & Molecular Immunology, 13 (2). pp. 251-262. ISSN 1672-7681

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Official URL: http://www.nature.com/cmi/journal/vaop/ncurrent/fu...


Increasing numbers of clinical trials and animal experiments have shown that probiotic bacteria are promising tools for allergy prevention. Here, we analyzed the immunomodulatory properties of three selected lactobacillus strains and the impact of their mixture on allergic sensitization to Bet v 1 using a gnotobiotic mouse model. We showed that Lactobacillus (L.) rhamnosus LOCK0900, L. rhamnosus LOCK0908 and L. casei LOCK0919 are recognized via Toll-like receptor 2 (TLR2) and nucleotide-binding oligomerization domain-containing protein 2 (NOD2) receptors and stimulate bone marrow-derived dendritic cells to produce cytokines in species- and strain-dependent manners. Colonization of germ-free (GF) mice with a mixture of all three strains (Lmix) improved the intestinal barrier by strengthening the apical junctional complexes of enterocytes and restoring the structures of microfilaments extending into the terminal web. Mice colonized with Lmix and sensitized to the Bet v 1 allergen showed significantly lower levels of allergen-specific IgE, IgG1 and IgG2a and an elevated total IgA level in the sera and intestinal lavages as well as an increased transforming growth factor (TGF)-β level compared with the sensitized GF mice. Splenocytes and mesenteric lymph node cells from the Lmix-colonized mice showed the significant upregulation of TGF-β after in vitro stimulation with Bet v 1. Our results show that Lmix colonization improved the gut epithelial barrier and reduced allergic sensitization to Bet v 1. Furthermore, these findings were accompanied by the increased production of circulating and secretory IgA and the regulatory cytokine TGF-β. Thus, this mixture of three lactobacillus strains shows potential for use in the prevention of increased gut permeability and the onset of allergies in humans.

Item Type:Article
Subjects:Q Science > QR Microbiology > QR180 Immunology
Divisions:Department of Microbial Biochemistry
ID Code:910
Deposited By: dr Tamara Aleksandrzak-Piekarczyk
Deposited On:29 Sep 2015 11:04
Last Modified:25 Apr 2016 09:36

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