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Newly identified protein Imi1 affects mitochondrial integrity and glutathione homeostasis in Saccharomyces cerevisiae

Kowalec, Piotr and Grynberg, Marcin and Pająk, Beata and Socha, Anna and Winiarska, Katarzyna and Fronk, Jan and Kurlandzka, Anna (2015) Newly identified protein Imi1 affects mitochondrial integrity and glutathione homeostasis in Saccharomyces cerevisiae. FEMS Yeast Research . ISSN 1567-1364 (In Press)

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Official URL: http://femsyr.oxfordjournals.org/

Abstract

Glutathione homeostasis is crucial for cell functioning. We describe a novel Imi1 protein of Saccharomyces cerevisiae affecting mitochondrial integrity and involved in controlling glutathione level. Imi1 is cytoplasmic and, except for its N-terminal Flo11 domain, has a distinct solenoid structure. A lack of Imi1 leads to mitochondrial lesions comprising aberrant morphology of cristae and multifarious mtDNA rearrangements and impaired respiration. The mitochondrial malfunctioning is coupled to significantly decrease of the level of intracellular reduced glutathione without affecting oxidized glutathione, which decreases the reduced/oxidized glutathione ratio. These defects are accompanied by decreased cadmium sensitivity and increased phytochelatin-2 level.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Q Science > QH Natural history > QH426 Genetics
Divisions:Department of Genetics
ID Code:913
Deposited By: dr hab. Anna Kurlandzka
Deposited On:29 Sep 2015 10:43
Last Modified:08 Mar 2018 15:33

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