Surmacz, Liliana and Wójcik, Jacek and Kania, Magdalena and Bentinger, Magnus and Danikiewicz, Witold and Dallner, Gustav and Surowiecki, Przemysław and Cmoch, Piotr and Swiezewska, Ewa (2015) Short-chain polyisoprenoids in the yeast Saccharomyces cerevisiae - new companions of the old guys. Biochimica et Biophysica Acta Molecular and Cell Biology of Lipids, 1851 (10). pp. 1296-1303. ISSN 10-1016
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Abstract
Dolichols are, among others, obligatory cofactors of protein glycosylation in eukaryotic cells. It is well known that yeast cells accumulate a family of dolichols with Dol-15/16 dominating while upon certain physiological conditions a second family with Dol-21 dominating is noted. In this report we identified the presence of additional short-chain length polyprenols - all-trans Pren-7 in three yeast strains (SS328, BY4741 and L5366), Pren-7 was accompanied by traces of putative Pren-6 and -8. Moreover, in two of these strains a single polyprenol mainly-cis-Pren-11 was synthesized at the stationary phase of growth. Identity of polyprenols was confirmed by HR-HPLC/MS, NMR and metabolic labeling. Additionally, simvastatin inhibited their biosynthesis.
| Item Type: | Article |
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| Uncontrolled Keywords: | polyisoprenoid alcohols; dolichol; prenol; Saccharomyces cerevisiae, yeast |
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Department of Lipid Biochemistry |
| ID Code: | 994 |
| Deposited By: | dr Liliana Surmacz |
| Deposited On: | 23 Oct 2015 09:26 |
| Last Modified: | 08 Mar 2018 15:33 |
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