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Shifts in rDNA levels act as a genome buffer promoting chromosome homeostasis

Deregowska, Anna and Adamczyk, Jagoda and Kwiatkowska, Aleksandra and Gurgul, Artur and Skoneczny, Marek and Skoneczna, Adrianna and Szmatola, Tomasz and Jasielczuk, Igor and Magda, Michał and Rawska, Ewa and Pabian, Sylwia and Panek, Anita and Kaplan, Jakub and Lewinska, Anna and Wnuk, Maciej (2015) Shifts in rDNA levels act as a genome buffer promoting chromosome homeostasis. Cell Cycle, 14 (21). pp. 3475-3487.


Official URL: http://dx.doi.org/10.1080/15384101.2015.1093705


The nucleolus is considered to be a stress sensor and rDNA-based regulation of cellular senescence and longevity has been proposed. However, the role of rDNA in the maintenance of genome integrity has not been investigated in detail. Using genomically diverse industrial yeasts as a model and array-based comparative genomic hybridization (aCGH), we show that chromosome level may be balanced during passages and as a response to alcohol stress that may be associated with changes in rDNA pools. Generation- and ethanol-mediated changes in genes responsible for protein and DNA/RNA metabolism were revealed using next-generation sequencing. Links between redox homeostasis, DNA stability, and telomere and nucleolus states were also established. These results suggest that yeast genome is dynamic and chromosome homeostasis may be controlled by rDNA.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Department of Genetics
Laboratory of Mutagenesis and DNA Repair
ID Code:1016
Deposited By: dr hab. Adrianna Skoneczna
Deposited On:01 Dec 2015 13:57
Last Modified:08 Mar 2018 15:33

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