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Dolichol phosphate mannose synthase from the pathogenic yeast Candida albicans is a multimeric enzyme.

Juchimiuk, Mateusz and Kruszewska, Joanna S. and Palamarczyk, Grazyna (2015) Dolichol phosphate mannose synthase from the pathogenic yeast Candida albicans is a multimeric enzyme. Biochimica et Biophysica Acta (BBA) - General Subjects, 1850 (11). pp. 2265-2275. ISSN 0304-4165

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Official URL: http://www.sciencedirect.com/science/article/pii/S...


Background: Dolichol phosphate mannose synthase (DPMS) is a key enzyme in N- and O-linked glycosylations and glycosylphosphatidylinositol (GPI)-anchor synthesis. DPMS generates DPM, the substrate for mentioned processes, by the transfer of mannosyl residue from GDP-Man to dolichol phosphate. Here we describe the role of DPMS for Candida albicans physiology with emphasis on the cell wall composition and morphogenesis. Methods: C. albicans genes for DPMS subunits were cloned, tagged and expressed in Saccharomyces cerevisiae. The C. albicans strains with controlled expression of DPM genes were constructed and analyzed. Gene expression and enzyme activities were measured using RT-PCR and radioactive substrate. Sensitivities against chemical agents were tested with microdilution method. The composition of the cell wall was estimated by HPLC. Glycosylation status of the marker protein was analyzed by Western blot. Morphological differentiation of the strains was checked on the media promoting hyphae and chlamydospore formation. Results: We demonstrate that C. albicans DPMS consists of three interacting subunits, among which Dpm1 and Dpm3 are indispensable, whereas Dpm2 increases enzymatic activity. Lowered expression of DPMS genes results in decreased DPMS activity, increased susceptibility to cell wall perturbing agents and in altered cell wall composition. Mutants Tetp-DPM1 and Tetp-DPM3 show defective protein glycosylation and are impaired in hyphae and chlamydospore formation. Major conclusion: DPMS from C. albicans, opposite to S. cerevisiae, belongs to the family of DPMS with multimeric protein structure. General significance: This work provides important data about factors required for a proper protein glycosylation and for morphogenesis of pathogenic yeast C. albicans.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QR Microbiology
Divisions:Laboratory of Fungal Glycobiology
ID Code:1049
Deposited By: prof dr hab Joanna S. Kruszewska
Deposited On:03 Dec 2015 14:31
Last Modified:08 Mar 2018 15:33

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