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Adenovirus Dodecahedron, a VLP, Can be Purified by Size Exclusion Chromatography Instead of Time-Consuming Sucrose Density Gradient Centrifugation.

Szurgot, Inga and Jedynak, M and Podsiadla-Bialoskorska, M and Piwowarski, Jan and Szolajska, Ewa and Chroboczek, Jadwiga (2015) Adenovirus Dodecahedron, a VLP, Can be Purified by Size Exclusion Chromatography Instead of Time-Consuming Sucrose Density Gradient Centrifugation. Molecular biotechnology, 57 (6). pp. 565-73. ISSN 1559-0305

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Official URL: http://link.springer.com/article/10.1007%2Fs12033-...

Abstract

Adenoviral dodecahedron (Dd) is a virus-like particle composed of twelve pentameric penton base (Pb) proteins, responsible for adenovirus cell penetration. It is generated spontaneously in the baculovirus system upon expression of the Pb gene of adenovirus serotype 3. This particle shows remarkable cell penetration ability with 2,00,000-3,00,000 Dd internalized into one cell in culture, conceivably delivering several millions of foreign cargo molecules to the target cell. We have used it in the past for delivery of small drugs as well as a vaccination platform, in which Dd serves as a particulate vaccine delivery system. Since development of new biomedicals depends strongly on the cost of their expression and purification, we attempted, albeit unsuccessfully, to obtain Dd expression in bacteria. We therefore retained its expression in the baculovirus/insect cells system but introduced significant improvements in the protocols for Dd expression and purification, leading to considerable savings in time and improved yield.

Item Type:Article
Subjects:Q Science > Q Science (General)
ID Code:1056
Deposited By: M.Sc. Marta Jedynak
Deposited On:07 Dec 2015 09:00
Last Modified:08 Mar 2018 15:33

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