IBB PAS Repository

Quantitative analysis of PPT1 interactome in human neuroblastoma cells.

Scifo, Enzo and Szwajda, Agnieszka and Soliymani, Rabah and Pezzini, Francesco and Bianchi, Marzia and Dapkunas, Arvydas and Dębski, Janusz and Uusi-Rauva, Kristiina and Dadlez, Michal and Gingras, Anne-Claude and Tyynelä, Jaana and Simonati, Alessandro and Jalanko, Anu and Baumann, Marc H and Lalowski, Maciej (2015) Quantitative analysis of PPT1 interactome in human neuroblastoma cells. Data in brief, 4 . pp. 207-16. ISSN 2352-3409

[img] Microsoft Word


Mutations in the CLN1 gene that encodes Palmitoyl protein thioesterase 1 (PPT1) or CLN1, cause Infantile NCL (INCL, MIM#256730). PPT1 removes long fatty acid chains such as palmitate from modified cysteine residues of proteins. The data shown here result from isolated protein complexes from PPT1-expressing SH-SY5Y stable cells that were subjected to single step affinity purification coupled to mass spectrometry (AP-MS). Prior to the MS analysis, we utilised a modified filter-aided sample preparation (FASP) protocol. Based on label free quantitative analysis of the data by SAINT, 23 PPT1 interacting partners (IP) were identified. A dense connectivity in PPT1 network was further revealed by functional coupling and extended network analyses, linking it to mitochondrial ATP synthesis coupled protein transport and thioester biosynthetic process. Moreover, the terms: inhibition of organismal death, movement disorders and concentration of lipid were predicted to be altered in the PPT1 network. Data presented here are related to Scifo et al. (J. Proteomics, 123 (2015) 42-53).

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Department of Biophysics
ID Code:1100
Deposited By: Mr Janusz Debski
Deposited On:22 Dec 2015 12:43
Last Modified:08 Mar 2018 15:33

Repository Staff Only: item control page