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Collagenase as a useful tool for the analysis of plant cellular peripheries

Luczak, Magdalena and Krzeszowiec-Jeleń, Weronika and Konopka-Postupolska, Dorota and Wojtaszek, Przemysław (2015) Collagenase as a useful tool for the analysis of plant cellular peripheries. Phytochemistry, 112 . pp. 195-209. ISSN 0031-9422

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Official URL: http://www.sciencedirect.com/science/article/pii/S...


A technique for the selective loosening of the cell wall structure and the isolation of proteins permanently knotted in the cell walls was elaborated. Following treatment with collagenase, some proteins, such as calreticulin (CRT) and auxin binding protein 1 (ABP1) were released from purified cell walls, most probably through destruction of respective interacting proteins. The results were confirmed by the immunolocalization of the ABP1 and CRT with confocal and electron microscopy. On the other hand, potential substrates of collagenase, among them annexin 1 have been recognized. Mass spectra of annexin 1 obtained after collagenase digestion and results from analysis of potential cleavage sites suggested that the mechanism of enzyme cleavage might not depend on the amino acid sequence. Summarizing, collagenase was found to be a very useful tool for exploring molecules involved in the functioning of cellular peripheries.

Item Type:Article
Subjects:Q Science > QH Natural history
Q Science > QH Natural history > QH301 Biology
Q Science > QK Botany
Divisions:Department of Plant Biochemistry
ID Code:1185
Deposited By: Dr Dorota Konopka-Postupolska
Deposited On:02 May 2016 09:32
Last Modified:02 May 2016 09:32

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