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A prime/boost vaccination with HA DNA and Pichia -produced HA protein elicits a strong humoral response in chickens against H5N1

Stachyra, Anna and Pietrzak, Maria and Macioła, Agnieszka and Protasiuk, Anna and Olszewska, Monika and Śmietanka, Krzysztof and Minta, Zenon and Góra-Sochacka, Anna and Kopera, Edyta and Sirko, Agnieszka (2017) A prime/boost vaccination with HA DNA and Pichia -produced HA protein elicits a strong humoral response in chickens against H5N1. Virus Research, 232 . pp. 41-47. ISSN 01681702

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Official URL: http://doi.org/10.1016/j.virusres.2017.01.025


Highly pathogenic avian influenza viruses cause severe disease and huge economic losses in domestic poultry and might pose a serious threat to people because of the high mortality rates in case of an accidental transmission to humans. The main goal of this work was to evaluate the immune responses and hemagglutination inhibition potential elicited by a combined DNA/recombinant protein prime/boost vaccination compared to DNA/DNA and protein/protein regimens in chickens. A plasmid encoding hemagglutinin (HA) from the A/swan/Poland/305-135V08/2006 (H5N1) virus, or the recombinant HA protein produced in Pichia pastoris system, both induced H5 HA–specific humoral immune responses in chickens. In two independent experiments, anti-HA antibodies were detected in sera collected two weeks after the first dose and the response was enhanced by the second dose of a vaccine, regardless of the type of subunit vaccine (DNA or recombinant protein) administered. The serum collected from chickens two weeks after the second dose was characterized by three types of assays: indirect ELISA, hemagglutination inhibition (HI) and a diagnostic test based on H5 antibody competition. Although the indirect ELISA failed to detect superiority of any of the three vaccine regimens, the other two tests clearly indicated that priming of chickens with the DNA vaccine significantly enhanced the protective potential of the recombinant protein vaccine produced in P. pastoris.

Item Type:Article
Subjects:Q Science > QR Microbiology > QR180 Immunology
Q Science > QR Microbiology > QR355 Virology
ID Code:1379
Deposited By: Anna Stachyra
Deposited On:11 Sep 2017 08:01
Last Modified:08 Mar 2018 15:34

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