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Identification of pig-specific Cryptosporidium species in mixed infections using Illumina sequencing technology

Kaupke, Agnieszka and Gawor, Jan and Rzeżutka, Artur and Gromadka, Robert (2017) Identification of pig-specific Cryptosporidium species in mixed infections using Illumina sequencing technology. Experimental Parasitology, 182 . pp. 22-25. ISSN 00144894

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Official URL: http://doi.org/10.1016/j.exppara.2017.09.020

Abstract

Nowadays molecular methods are widely used in epidemiological studies of Cryptosporidium infections in humans and animals. However to gain better understanding of parasite species or genotypes, especially when mixed infections are noticed, highly sensitive tools with adequate resolution power need to be employed. In this article, we report an application of the next generation sequencing method (NGS) for detection and characterisation of Cryptosporidium species concurrently present in pig faeces. A mixture of Cryptosporidium DNA obtained from two faecal samples was amplified at the 18 SSU rRNA gene locus and the resulting amplicons were subsequently used for MiSeq sequencing. Although initial molecular analyses indicated the possible presence of another Cryptosporidium species other than Cryptosporidium scrofarum and Cryptosporidium suis, deep sequencing only confirmed the presence of pig-specific Cryptosporidium.

Item Type:Article
Subjects:Q Science > QH Natural history > QH426 Genetics
Q Science > QL Zoology
Divisions:Laboratory of DNA Sequencing and Oligonucleotide Synthesis
ID Code:1392
Deposited By: Robert Gromadka
Deposited On:08 Nov 2017 10:56
Last Modified:08 Nov 2017 10:56

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