Stasiak, Marta and Gawryś, Katarzyna and Popielarsk, Marcin and Bednarek, Radosław and Studzian, Maciej and Sitkiewicz, Ewa and Szemraj, Janusz and Swiatkowska, Maria (2017) Differential Quantitative Proteomics of Human Microvascular Endothelial Cells 1 by iTRAQ Reveals Palladin to be a New Biomarker During TGF-β1 Induced Endothelial Mesenchymal Transition. Journal of Proteomics & Bioinformatics, 10 . pp. 236-245. ISSN 0974-276X (In Press)
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Official URL: https://www.omicsonline.org/open-access/differenti...
Abstract
The study uses global quantitative proteomics to investigate the molecular mechanisms behind the induction of endothelial-mesenchymal transition (EndMT) by transforming growth factor–β (TGF-β). Orbitrap Velos mass spectrometers and iTRAQ – a labeling-based analysis were used to perform a global and quantitative comparison of two proteomes of Human Microvascular Endothelial Cells-1 (HMEC-1) treated or not treated by TGF-β1. iTRAQ analysis identified 43 differentially-expressed proteins in the early stages of EndMT induced by TGF-β1. From 5522 identified proteins, 26 were downregulated and 17 were upregulated, including proteins such as palladin, POTE I, torsin A and nucleoporin (NDC1). Further analysis of palladin revealed its increased mRNA and protein expression in response to TGF-β and Snail transcription factor. Our findings demonstrate that the newly- identified proteins may be involved in early stages of biological processes leading to EndMT
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > Q Science (General) |
| Divisions: | Mass Spectrometry Laboratory |
| ID Code: | 1414 |
| Deposited By: | dr Ewa Sitkiewicz |
| Deposited On: | 09 Nov 2017 09:49 |
| Last Modified: | 09 Nov 2017 09:49 |
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