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Fine-tuning of the stability of β-strands by Y181 in perfringolysin O directs the prepore to pore transition

Kulma, Magdalena and Kacprzyk-Stokowiec, Aleksandra and Traczyk, Gabriela and Kwiatkowska, Katarzyna and Dadlez, Michal (2018) Fine-tuning of the stability of β-strands by Y181 in perfringolysin O directs the prepore to pore transition. Biochimica et Biophysica Acta (BBA) - Biomembranes . ISSN 00052736 (In Press)

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Official URL: http://doi.org/10.1016/j.bbamem.2018.08.008


Perfringolysin O (PFO) is a toxic protein that forms β-barrel transmembrane pores upon binding to cholesterol-containing membranes. The formation of lytic pores requires conformational changes in PFO that lead to the conversion of water-soluble monomers into membrane-bound oligomers. Although the general outline of stepwise pore formation has been established, the underlying mechanistic details await clarification. To extend our understanding of the molecular mechanisms that control the pore formation, we compared the hydrogen-deuterium exchange patterns of PFO with its derivatives bearing mutations in the D3 domain. In the case of two of these mutations F318A, Y181A, known from previous work to lead to a decreased lytic activity, global destabilization of all protein domains was observed in their water-soluble forms. This was accompanied by local changes in D3 β-sheet, including unexpected stabilization of functionally important β1 strand in Y181A. In case of the double mutation (F318A/Y181A) that completely abolished the lytic activity, several local changes were retained, but the global destabilization effects of single mutations were reverted and hydrogen-deuterium exchange (HDX) pattern returned to PFO level. Strong structural perturbations were not observed in case of remaining variants in which other residues of the hydrophobic core of D3 domain were substituted by alanine. Our results indicate the existence in PFO of a well-tuned H-bonding network that maintains the stability of the D3 β-strands at appropriate level at each transformation step. F318 and Y181 moieties participate in this network and their role extends beyond their direct intermolecular interaction during oligomerization that was identified previously.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Mass Spectrometry Laboratory
ID Code:1608
Deposited By: Magdalena Kulma
Deposited On:31 Oct 2018 08:05
Last Modified:31 Oct 2018 08:05

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