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A structural model of the immune checkpoint CD160-HVEM complex derived from HDX-mass spectrometry and molecular modeling

Kuncewicz, Katarzyna and Spodzieja, Marta and Sieradzan, Adam and Karczyńska, Agnieszka and Dąbrowska, Katarzyna and Dadlez, Michal and Speiser, Daniel E. and Derre, Laurent and Rodziewicz-Motowidło, Sylwia (2019) A structural model of the immune checkpoint CD160-HVEM complex derived from HDX-mass spectrometry and molecular modeling. Oncotarget, 10 (4). ISSN 1949-2553

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Official URL: http://doi.org/10.18632/oncotarget.26570

Abstract

CD160 is a T cell coinhibitory molecule that interacts with the herpes virus entry mediator (HVEM) on antigen-presenting cells to provide an inhibitory signal to T cells. To date, the structure of CD160 and its complex with HVEM are unknown. Here, we have identified the fragments of CD160 interacting with HVEM using ELISA tests, hydrogen/deuterium studies, affinity chromatography and mass spectrometry (MS). By combining hydrogen/deuterium exchange and mass spectrometry (HDX-MS) we obtained key information about the tertiary structure of CD160, predicting the 3D structure of the CD160-HVEM complex. Our results provide insights into the molecular architecture of this complex, serving as a useful basis for designing inhibitors for future immunotherapies.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Mass Spectrometry Laboratory
ID Code:1767
Deposited By: Katarzyna Dąbrowska
Deposited On:25 Nov 2019 11:01
Last Modified:25 Nov 2019 11:01

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