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Enhanced oligomerization of full-length RAGE by synergy of the interaction of its domains

Moysa, Alexander and Hammerschmid, Dietmar and Szczepanowski, Roman H. and Sobott, Frank and Dadlez, Michal (2019) Enhanced oligomerization of full-length RAGE by synergy of the interaction of its domains. Scientific Reports, 9 (1). p. 20332. ISSN 2045-2322

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Official URL: http://doi.org/10.1038/s41598-019-56993-9

Abstract

The pattern recognition receptor RAGE (receptor for advanced glycation end-products) transmits proinflammatory signals in several inflammation-related pathological states, including vascular diseases, cancer, neurodegeneration and diabetes. Its oligomerization is believed to be important in signal transduction, but RAGE oligomeric structures and stoichiometries remain unclear. Different oligomerization modes have been proposed in studies involving different truncated versions of the extracellular parts of RAGE. Here, we provide basic characterization of the oligomerization patterns of full-length RAGE (including the transmembrane (TM) and cytosolic regions) and compare the results with oligomerization modes of its four truncated fragments. For this purpose, we used native mass spectrometry, analytical ultracentrifugation, and size-exclusion chromatography coupled with multi-angle light scattering. Our results confirm known oligomerization tendencies of separate domains and highlight the enhanced oligomerization properties of full-length RAGE. Mutational analyses within the GxxxG motif of the TM region show sensitivity of oligomeric distributions to the TM sequence. Using hydrogen-deuterium exchange, we mapped regions involved in TM-dependent RAGE oligomerization. Our data provide experimental evidence for the major role of the C2 and TM domains in oligomerization, underscoring synergy among different oligomerization contact regions along the RAGE sequence. These results also explain the variability of obtained oligomerization modes in RAGE fragments.

Item Type:Article
Subjects:Q Science > Q Science (General)
Divisions:Mass Spectrometry Laboratory
ID Code:1821
Deposited By: PhD Alexander Moysa
Deposited On:03 Feb 2020 10:01
Last Modified:03 Feb 2020 10:01

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