IBB PAS Repository

Selected ALKBH dioxygenases are overexpressed in salivary gland tumours.

Marcinkowski, Michał and Garbicz, Damian and Pilżys, Tomasz and Kukwa, Wojciech and Grzesiuk, Elzbieta (2022) Selected ALKBH dioxygenases are overexpressed in salivary gland tumours. Acta Biochimica Polonica, 69 (4). pp. 889-894. ISSN 1734-154X


Official URL: https://ojs.ptbioch.edu.pl/index.php/abp/article/v...


Salivary gland tumours (SGTs) are a heterogeneous group of benign tumours of various origins and pathologies, showing a number of DNA modifications. Previously, in malignant head and neck cancer (HNSCC), we found overexpression of ALKBH proteins, the homologs of Escherichia coli AlkB 2-oxoglutarate and Fe(II) dependent dioxygenase. Moreover, we proved the connection of some of these dioxygenases with cancer development. Here, we studied the expression of five of these ALKBH dioxygenases: 1, 3, 4, 5, and FTO in benign SGTs. Using Western blot analysis, we found overexpression of three proteins: ALKBH1, 4, and FTO in SGT as compared to the surrounding, unaffected tissue. ALKBH4 was overexpressed in 76% of patient samples, whereas ALKBH1 and FTO in 65% of the samples. These results differ from those obtained in HNSCC, where FTO overexpression has been observed in 90% of patient samples. We also investigated the relationships between ALKBHs’ expression levels in normal and SGT tissues and identified two correlated pairs: ALKBH1-ALKBH3 and ALKBH1-ALKBH5. Additionally, in tumour tissue ALKBHs: ALKBH1, ALKBH3, ALKBH4, and ALKBH5 levels were correlated with each other. Together, these findings show that the ALKBH proteins exhibit pro cancerogenic action in SGT, even though the levels ALKBHs are generally lower in benign SGT than in malignant HNSCC. We suggest that the overexpression of the ALKBHs, especially FTO, may be used as a cancer marker and for its grading.

Item Type:Article
Subjects:Q Science > Q Science (General)
R Medicine > R Medicine (General)
R Medicine > RB Pathology
Divisions:Department of Molecular Biology
ID Code:2198
Deposited By: Prof. Elżbieta Grzesiuk
Deposited On:19 Dec 2022 14:32
Last Modified:19 Dec 2022 14:32

Repository Staff Only: item control page