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Sugar Starvation Disrupts Lipid Breakdown by Inducing Autophagy in Embryonic Axes of Lupin (Lupinus spp.) Germinating Seeds

Borek, Sławomir and Stefaniak, Szymon and Nuc, Katarzyna and Wojtyla, Łukasz and Ratajczak, Ewelina and Sitkiewicz, Ewa and Malinowska, Agata and Świderska, Bianka and Wleklik, Karolina and Pietrowska-Borek, Małgorzata (2023) Sugar Starvation Disrupts Lipid Breakdown by Inducing Autophagy in Embryonic Axes of Lupin (Lupinus spp.) Germinating Seeds. International Journal of Molecular Sciences, 24 (14). p. 11773. ISSN 1422-0067

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Official URL: http://doi.org/10.3390/ijms241411773


Under nutrient deficiency or starvation conditions, the mobilization of storage compounds during seed germination is enhanced to primarily supply respiratory substrates and hence increase the potential of cell survival. Nevertheless, we found that, under sugar starvation conditions in isolated embryonic axes of white lupin (Lupinus albus L.) and Andean lupin (Lupinus mutabilis Sweet) cultured in vitro for 96 h, the disruption of lipid breakdown occurs, as was reflected in the higher lipid content in the sugar-starved (-S) than in the sucrose-fed (+S) axes. We postulate that pexophagy (autophagic degradation of the peroxisome—a key organelle in lipid catabolism) is one of the reasons for the disruption in lipid breakdown under starvation conditions. Evidence of pexophagy can be: (i) the higher transcript level of genes encoding proteins of pexophagy machinery, and (ii) the lower content of the peroxisome marker Pex14p and its increase caused by an autophagy inhibitor (concanamycin A) in -S axes in comparison to the +S axes. Additionally, based on ultrastructure observation, we documented that, under sugar starvation conditions lipophagy (autophagic degradation of whole lipid droplets) may also occur but this type of selective autophagy seems to be restricted under starvation conditions. Our results also show that autophagy occurs at the very early stages of plant growth and development, including the cells of embryonic seed organs, and allows cell survival under starvation conditions.

Item Type:Article
Subjects:Q Science > QK Botany
Q Science > QP Physiology
Divisions:Mass Spectrometry Laboratory
ID Code:2299
Deposited By: Bianka Świderska
Deposited On:04 Aug 2023 07:18
Last Modified:09 Aug 2023 10:01

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