Malecka, Kamila and Grabowska, Iwona and Radecki, Jerzy and Stachyra, Anna and Góra-Sochacka, Anna and Sirko, Agnieszka and Radecka, Hanna (2012) Voltammetric Detection of a Specific DNA Sequence of Avian Influenza Virus H5N1 Using HS-ssDNA Probe Deposited onto Gold Electrode. Electroanalysis, 24 (2). pp. 439-446. ISSN 10400397
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Official URL: http://dx.doi.org/10.1002/elan.201100566
Abstract
The working principle of genosensor relay on the mechanism of ion–channel mimetic sensor. The analytical signals generated upon hybridization processes were recorded by redox active marker [Fe(CN)6]3-/4- present in the sample solution using voltammetric techniques. The developed genosensor was suitable for determination of 20-mer complementary oligonucleotide sequence, and also of PCR products containing the complementary 20-mer sequence in various positions, with detection limits in 10 pM range. Non-complementary 20-mer oligonucleotide sequence as well as PCR product without complementary region generated very weak response. The good discrimination of the position of complementary part in the PCR products was observed
| Item Type: | Article |
|---|---|
| Subjects: | Q Science > QD Chemistry T Technology > TP Chemical technology |
| Divisions: | Department of Protein Biosynthesis |
| ID Code: | 371 |
| Deposited By: | dr Anna Gora-Sochacka |
| Deposited On: | 01 Jan 2013 19:42 |
| Last Modified: | 01 Jan 2013 19:42 |
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