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Synthetic genistein derivatives as modulators of glycosaminoglycan synthesis

Kloska, Anna and Narajczyk, Magdalena and Jakóbkiewicz-Banecka, Joanna and Grynkiewicz, Grzegorz and Szeja, Władysław and Gabig-Cimińska, Magdalena and Węgrzyn, Grzegorz (2012) Synthetic genistein derivatives as modulators of glycosaminoglycan synthesis. Journal of Translational Medicine, 10 . pp. 153-163.

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Background: Mucopolysaccharidoses (MPS) are severe metabolic disorders caused by 26 accumulation of undegraded glycosaminoglycans (GAGs) in lysosomes due to defects in certain 27 lysosomal hydrolases. Substrate reduction therapy (SRT) has been proposed as one of potential 28 treatment procedures of MPS. Importantly, small molecules used in such a therapy might 29 potentially cross the blood-brain barrier (BBB) and improve neurological status of patients, as 30 reported for a natural isoflavone, 5, 7-dihydroxy-3- (4-hydroxyphenyl)-4H-1-benzopyran-4-one, 31 also known as genistein. Although genistein is able to cross BBB to some extent, its delivery to 32 the central nervous system is still relatively poor (below 10% efficiency). Thus, we aimed to 33 develop a set of synthetically modified genistein molecules and characterize physicochemical as 34 well as biological properties of these compounds. Methods: Following parameters were 35 determined for the tested synthetic derivatives of genistein: cytotoxicity, effects on cell 36 proliferation, kinetics of GAG synthesis, effects on epidermal growth factor (EGF) receptor’s 37 tyrosine kinase activity, effects on lysosomal storage, potential ability to cross BBB. Results: We 38 observed that some synthetic derivatives inhibited GAG synthesis similarly to, or more 39 efficiently than, genistein and were able to reduce lysosomal storage in MPS III fibroblasts. The 40 tested compounds were generally of low cytotoxicity and had minor effects on cell proliferation. 41 Moreover, synthetic derivatives of genistein revealed higher lipophilicity (assessed in silico) than 42 the natural isoflavone. Conclusion: Some compounds tested in this study might be promising 43 candidates for further studies on therapeutic agents in MPS types with neurological symptoms.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history > QH426 Genetics
R Medicine > R Medicine (General)
Divisions:Laboratory of Molecular Biology (in Gdansk)
ID Code:383
Deposited By: Prof. Magdalena Gabig-Cimińska
Deposited On:03 Apr 2013 09:04
Last Modified:03 Apr 2013 09:04

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