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Simultaneous siRNA-mediated silencing of pairs of genes coding for enzymes involved in glycosaminoglycan synthesis

Dziedzic, Dariusz and Narajczyk, Magdalena and Gabig-Cimińska, Magdalena and Jakóbkiewicz-Banecka, Joanna (2012) Simultaneous siRNA-mediated silencing of pairs of genes coding for enzymes involved in glycosaminoglycan synthesis. Acta Biochimica Polonica, 59 (2). pp. 293-298.

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Abstract

It has been demonstrated recently that it is possible to decrease expression of genes coding for enzymes involved in synthesis of glycosaminoglycans (GAGs) by using specific siRNAs which interfere with stability of particular mRNAs. This procedure has been proposed as a potential treatment for patients suffering from mucopolysaccharidoses, a group of inherited metabolic diseases caused by dysfunction of enzymes required for GAG degradation, and resultant storage of these compounds in cells of affected persons. Here, we asked if the simultaneous use two species of specific siRNAs aimed at silencing two genes involved in particular steps of GAG synthesis may be more effective than the use of single siRNA. We found that inhibition of GAG synthesis in cells treated with two siRNAs is generally more effective than using single siRNAs. However, the differences were not statistically significant, therefore the potential benefit from the use of two siRNAs over the use of a single siRNA is doubtful in the light of the cost-benefit ratio and possibly stronger side-effects of the putative therapy.

Item Type:Article
Subjects:Q Science > Q Science (General)
Q Science > QH Natural history > QH426 Genetics
Divisions:Laboratory of Molecular Biology (in Gdansk)
ID Code:385
Deposited By: Prof. Magdalena Gabig-Cimińska
Deposited On:14 Apr 2014 11:55
Last Modified:14 Apr 2014 11:55

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