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Method and platform standardization in MRM-based quantitative plasma proteomics.

Percy, Andrew J and Chambers, Andrew G and Yang, Juncong and Jackson, Angela M and Domanski, Dominik and Burkhart, Julia and Sickmann, Albert and Borchers, Christoph H (2013) Method and platform standardization in MRM-based quantitative plasma proteomics. Journal of proteomics, 95 . pp. 66-76. ISSN 1876-7737

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Official URL: http://www.sciencedirect.com/science/article/pii/S...


There exists a growing demand in the proteomics community to standardize experimental methods and liquid chromatography-mass spectrometry (LC/MS) platforms in order to enable the acquisition of more precise and accurate quantitative data. This necessity is heightened by the evolving trend of verifying and validating candidate disease biomarkers in complex biofluids, such as blood plasma, through targeted multiple reaction monitoring (MRM)-based approaches with stable isotope-labeled standards (SIS). Considering the lack of performance standards for quantitative plasma proteomics, we previously developed two reference kits to evaluate the MRM with SIS peptide approach using undepleted and non-enriched human plasma. The first kit tests the effectiveness of the LC/MRM-MS platform (kit #1), while the second evaluates the performance of an entire analytical workflow (kit #2). Here, these kits have been refined for practical use and then evaluated through intra- and inter-laboratory testing on 6 common LC/MS platforms. For an identical panel of 22 plasma proteins, similar concentrations were determined, regardless of the kit, instrument platform, and laboratory of analysis. These results demonstrate the value of the kit and reinforce the utility of standardized methods and protocols.

Item Type:Article
Subjects:Q Science > Q Science (General)
ID Code:712
Deposited By: Dr Dominik Domanski
Deposited On:17 Jun 2014 08:35
Last Modified:27 Oct 2014 14:48

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