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Analysis of plant polyisoprenoids

Gawarecka, Katarzyna and Swiezewska, Ewa (2014) Analysis of plant polyisoprenoids. Methods in Molecular Biology, 1153 . pp. 135-147. ISSN ISBN 978-1-4939-0605-5

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Official URL: http://link.springer.com/protocol/10.1007%2F978-1-...


Polyisoprenoid alcohols are representatives of high-molecular terpenoids. Their hydrocarbon chains are built of 5 to more than 100 isoprene units giving rise to polymer molecules that differ in chain-length and/or geometrical configuration. Plants have been shown to accumulate diverse polyisoprenoid mixtures with tissue-specific composition. In this chapter, methods of analysis of polyisoprenoid alcohols in plant material are described, including isolation and purification of polyisoprenoids from plant tissue, fast semiquantitative analysis of the polyisoprenoid profile by thin-layer chromatography (straight phase adsorption and reversed phase partition techniques), and quantification of polyisoprenoids with the aid of high performance liquid chromatography. This approach results in full characterization of complex polyisoprenoid mixtures accumulated in various plant tissues and other matrixes.

Item Type:Article
Subjects:Q Science > QH Natural history > QH301 Biology
Divisions:Department of Lipid Biochemistry
ID Code:751
Deposited By: Ewa Swiezewska
Deposited On:18 Dec 2014 10:40
Last Modified:08 Mar 2018 15:33

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