Self-adjuvanting influenza candidate vaccine presenting epitopes forcell-mediated immunity on a proteinaceous multivalentnanoplatform

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Abstract

We exploit the features of a virus-like particle, adenoviral dodecahedron (Ad Dd), for engineering amultivalent vaccination platform carrying influenza epitopes for cell-mediated immunity. The deliveryplatform, Ad Dd, is a proteinaceous, polyvalent, and biodegradable nanoparticle endowed with remark-able endocytosis activity that can be engineered to carry 60 copies of a peptide. Influenza M1 is the mostabundant influenza internal protein with the conserved primary structure. Two different M1 immuno-dominant epitopes were separately inserted in Dd external positions without destroying the particles’dodecahedric structure. Both kinds of DdFluM1 obtained through expression in baculovirus system wereproperly presented by human dendritic cells triggering efficient activation of antigen-specific T cellsresponses. Importantly, the candidate vaccine was able to induce cellular immunity in vivo in chickens.These results warrant further investigation of Dd as a platform for candidate vaccine, able to stimulatecellular immune responses.

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• Self-adjuvanting influenza candidate vaccine presenting epitopes forcell-mediated immunity on a proteinaceous multivalentnanoplatform. (deposited UNSPECIFIED)
  • Self-adjuvanting influenza candidate vaccine presenting epitopes forcell-mediated immunity on a proteinaceous multivalentnanoplatform. (deposited 14 Apr 2014 10:16) [Currently Displayed]